Xanthine Oxidase-Derived ROS Display a Biphasic Effect on Endothelial Cells Adhesion and FAK Phosphorylation

Figure 3

Hx-XO-derived ROS induced time-dependent dephosphorylation of ERK1/2. HUVECs were treated with Hx-XO (4.5 mU/mL) for the indicated times after which cells were harvested and the soluble fraction analysed for ERK1/2 phosphorylation. Western blot analysis with anti-phospho-ERK1/2 was performed as described in Materials and Methods. Total ERK1/2 proteins levels at all times points were equivalent as measured by immunoblot of the same membranes using antibodies against total ERK1/2. (b) Phosphorylated ERK1/2 from different experiments was quantified by densitometry and corrected for the amount of ERK1/2 which was quantified by densitometry. Results are expressed as mean ± SEM, . versus untreated cells.

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