PAN-811 Blocks Chemotherapy Drug-Induced In Vitro Neurotoxicity, While Not Affecting Suppression of Cancer Cell Growth

Figure 3

Suppression of 5-FU/MTX-induced increases in LDH and DHR123 readings by PAN-811. (a) LDH release analysis for neurons that were cultured in 17.5% AOs-containing medium and insulted with both 100 μM MTX and 25 μM 5-FU in the absence or presence of PAN-811⋅Cl⋅H₂O at different concentrations for 1 day (blue bars ); Green bar represents noninsult/untreated control. (b) Fluorescent microscope for neurons in 17.5% AOs-containing medium insulted with both 100 μM MTX and 25 μM 5-FU in the absence or presence of 10 μM PAN-811⋅Cl⋅H₂O for 1 day and incubated with DHR123 for 30 min (bar = 50 μm). (c) Quantification of (b) at excitation at 485 nm and emission at 520 nm (). Data are expressed as % suppression = [(Insulted&Untreated − Insulted&Treated)/(Insulted&Untreated − NonInsulted&Untreated) 100%]. Figure symbols are , < 0.05, compared with insult group alone (without PAN-811 treatment) by Student’s -test (one tail) and one-factor ANOVA followed by Tukey HSD test; , ; , , compared with noninsult/untreated control group by one-factor ANOVA followed with Tukey HSD test.

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