The Protective Roles of ROS-Mediated Mitophagy on ¹²⁵I Seeds Radiation Induced Cell Death in HCT116 Cells

Figure 1

Inhibition of autophagy enhanced the sensitivity of HCT116 cells to ¹²⁵I seeds radiation. (a) HCT116 cells were pretreated with or without 3-MA before exposure to the indicated dose of ¹²⁵I seeds radiation. Clonogenic survival was assessed in the presence or and absence of 3-MA. (b) Clonogenic survival was assessed in HCT116 cells pretreated with or without Ly294002 before exposure to the indicated dose of ¹²⁵I seeds radiation. (c) HCT116 cells were transfected with LC3 siRNA or unrelated control siRNA. Western blot was performed to assess the expression of LC3 48 h after transfection. Representative Western blot analysis of LC3II/I is shown. (d) Clonogenic survival was assessed in HCT116 cells transfected with LC3 siRNA or unrelated control siRNA before exposure to the indicated dose of ¹²⁵I seeds radiation. The values are the means ± SD of three independent experiments. The two-tailed -test was used for comparing the means. indicates significant difference () as compared to the control group.

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