The Protective Roles of ROS-Mediated Mitophagy on ¹²⁵I Seeds Radiation Induced Cell Death in HCT116 Cells

Figure 6

¹²⁵I seeds radiation induced oxidative damage in mitochondria. HCT116 cells were exposed to 2 Gy of ¹²⁵I seeds radiation. (a) The cells were fixed and stained with anti-Tim23 antibody, and images were captured by a confocal microscope. The representative images are shown (scale bar 10 μm). (b) The mitochondrial potential was analyzed by flow cytometry with a JC-1 probe. Typical example of flow cytometry analysis was shown. (c) The ratio of J-aggregates and JC-1 monomers was used to evaluate the mitochondrial potential. The values are the means ± SD of three independent experiments. (d) Total ATP was evaluated by an ATP assay kit. The values are the means ± SD of three independent experiments. (e) Mitochondrial ROS were analyzed by flow cytometry after staining with MitoSOX. Typical example of flow cytometry analysis was shown. (f) Quantitative analysis of mitochondrial ROS. The values were derived from mean fluorescence intensity. The values are the means ± SD of three independent experiments. (g) Intracellular ROS were stained by DCFH-DA and analyzed by flow cytometry. (h) Quantitative analysis of intracellular ROS. The values were derived from mean fluorescence intensity. The values are the means ± SD of three independent experiments. , as compared to the control group.

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