Research Article
Effects of Astragalus Polysaccharides on Dysfunction of Mitochondrial Dynamics Induced by Oxidative Stress
Table 1
Primers and conditions used for RT-PCR.
| | Gene | Forward primer (5′-3′),
reverse primer (5′-3′) | -An. (°C) | Gene | Forward primer (5′-3′),
reverse primer (5′-3′) | -An. (°C) |
| | Mfn-1 | ATGGCAGAAACGGTATCTCCA
CTCGGATGCTATTCGATCAAGTT | 60.5 | Drp-1 | CAGGAATTGTTACGGTTCCCTAA
CCTGAATTAACTTGTCCCGTGA | 60 |
| | Mfn-2 | CTGGGGACCGGATCTTCTTC
CTGCCTCTCGAAATTCTGAAACT | 61 | Fis-1 | TGTCCAAGAGCACGCAATTTG
CCTCGCACATACTTTAGAGCCTT | 62 |
| | Opa-1 | TGGAAAATGGTTCGAGAGTCAG
CATTCCGTCTCTAGGTTAAAGCG | 60.5 | GAPDH | TGGATTTGGACGCATTGGTC
TTTGCACTGGTACGTGTTGAT | 60.5 |
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-An.: annealing temperature; Mfn-1: mitofusin 1; Mfn-2: mitofusin 2; Opa-1: optic atrophy 1; Drp-1: dynamin-related protein q; Fis-1: mitochondrial fission 1 protein.
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