Nr2e1 Deficiency Augments Palmitate-Induced Oxidative Stress in Beta Cells

Figure 5

Nr2e1 silencing exaggerates lipotoxicity-induced beta cell dysfunction. (a) Insulin secretion in response to 2.5 and 20 mM glucose in MIN6-shc, MIN6-sh2, and MIN6-sh3 cells preincubated during 48 h with or without 0.5 mM palmitate. Results are means ± SD of 3 independent experiments. compared to MIN6-shc in 2.5 mM glucose. compared to MIN6-shc in 20 mM glucose. (b) Insulin mRNA levels of MIN6-shc, MIN6-sh2, and MIN6-sh3 cells incubated during 48 h with or without 0.5 mM palmitate. Data represent means ± SD of three observations. compared to MIN6-shc incubated with palmitate.

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