Nr2e1 Deficiency Augments Palmitate-Induced Oxidative Stress in Beta Cells

Figure 6

Nr2e1 silencing affects oxidative stress and antioxidant enzyme levels. (a) Comparison of intracellular levels of ROS between control (MIN6-shc) and Nr2e1 silenced (MIN6-sh2 and MIN6-sh3) cells. Cells were cultured in complete medium with or without 0.5 mM palmitate for 24 h and then the fluorescence intensity of DHE was measured using flow cytometry. Data represent means ± SD of three observations; compared to cells in normal conditions. compared to MIN6-shc cells. (b) SOD and GSH-Px activity decreased in MIN6-sh2 and MIN6-sh3 cells. (c) GSH content decreased in MIN6-sh2 and MIN6-sh3 cells. (d) The relative mRNA expressions of Sod1, Gpx, Gclc, and Gclm were downregulated by Nr2e1 silencing. Data represent means ± SD of three observations; ; all comparisons with MIN6-shc cells.

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