Reinforced Epithelial Barrier Integrity via Matriptase Induction with Sphingosine-1-Phosphate Did Not Result in Disturbances in Physiological Redox Status

Figure 2

(a) Transepithelial electrical resistance values of IPEC-J2 cell monolayers after 0.5, 2, 24, and 48 hr treatment with S1P (200 ng/mL) and suramin (50 μM) were measured and compared to controls in nine parallel experiments. At the start of the treatment IPEC-J2 cells were not completely differentiated. The values are expressed in average relative TERs ± SEMs. Asterisk indicates significant differences between treated and mock groups (, , and ). (b) Transepithelial electrical resistance values of differentiated IPEC-J2 cell monolayers after 0.5, 2 hr treatment with S1P (200 ng/mL) and suramin (50 μM) were measured and compared to controls in nine parallel experiments. The values are expressed in average relative TERs ± SEMs. Asterisk indicates significant differences between treated and mock groups ( and ).

(a)

(b)