Research Article
Downregulation of Glutathione Biosynthesis Contributes to Oxidative Stress and Liver Dysfunction in Acute Kidney Injury
Figure 9
Glutathione measurement and siRNA transfection in HepG2. Cells were incubated in the absence (control) or presence of DL-propargylglycine (PAG) for 16 h and total glutathione was measured (a). Cells were transfected with Nrf2 siRNA or scrambled siRNA (control). The mRNA of Nrf2 (b) and glutamate-cysteine ligase subunits Gclc (c) and Gclm (d) were determined. Results are expressed as mean ± SE ( for each group). when compared with the value obtained from control cells.
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