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Oxidative Medicine and Cellular Longevity
Volume 2016, Article ID 9785890, 19 pages
Research Article

Metformin Decreases Reactive Oxygen Species, Enhances Osteogenic Properties of Adipose-Derived Multipotent Mesenchymal Stem Cells In Vitro, and Increases Bone Density In Vivo

1Electron Microscopy Laboratory, Wroclaw University of Environmental and Life Sciences, 5b Kozuchowska Street, 51-631 Wroclaw, Poland
2Wroclaw Research Centre EIT+, 147 Stablowicka Street, 54-066 Wroclaw, Poland
3Department of Anatomy, Jagiellonian University Medical College, 12 Kopernika Street, 31-034 Krakow, Poland
4Faculty of Physics and Applied Computer Science, AGH University of Science and Technology, 30 Mickiewicza Street, 30059 Krakow, Poland
5Department of Animal Physiology and Biostructure, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, 31 Norwida Street, 50-375 Wroclaw, Poland

Received 28 December 2015; Revised 24 March 2016; Accepted 30 March 2016

Academic Editor: Ange Mouithys-Mickalad

Copyright © 2016 Krzysztof Marycz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Due to its pleiotropic effects, the commonly used drug metformin has gained renewed interest among medical researchers. While metformin is mainly used for the treatment of diabetes, recent studies suggest that it may have further application in anticancer and antiaging therapies. In this study, we investigated the proliferative potential, accumulation of oxidative stress factors, and osteogenic and adipogenic differentiation potential of mouse adipose-derived stem cells (MuASCs) isolated from mice treated with metformin for 8 weeks. Moreover, we investigated the influence of metformin supplementation on mice bone density and bone element composition. The ASCs isolated from mice who were treated with metformin for 8 weeks showed highest proliferative potential, generated a robust net of cytoskeletal projections, had reduced expression of markers associated with cellular senescence, and decreased amount of reactive oxygen species in comparison to control group. Furthermore, we demonstrated that these cells possessed greatest osteogenic differentiation potential, while their adipogenic differentiation ability was reduced. We also demonstrated that metformin supplementation increases bone density in vivo. Our result stands as a valuable source of data regarding the in vivo influence of metformin on ASCs and bone density and supports a role for metformin in regenerative medicine.