Metformin Decreases Reactive Oxygen Species, Enhances Osteogenic Properties of Adipose-Derived Multipotent Mesenchymal Stem Cells In Vitro, and Increases Bone Density In Vivo

Figure 9

Evaluation of adipogenic potential. Growth kinetics and morphology of cells cultured under adipogenic culture conditions. Proliferation factor (a), population doubling time (b). Morphology evaluation after 12 days of culture (c). Nuclei staining with DAPI (I, IV), phalloidin (II, V), and Oil Red O (III, VI). Magnifications: DAPI/phalloidin, ×100, scale bars: 250 μm; Oil Red O ×50, scale bar: 500 μm. Oil Red O staining was quantified by calculation percentage of stained area (d). Moreover, using RT-PCR mRNA levels of Adiponectin were assessed (e). Results expressed as mean ± SD. value < 0.05, .

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