Metformin Decreases Reactive Oxygen Species, Enhances Osteogenic Properties of Adipose-Derived Multipotent Mesenchymal Stem Cells In Vitro, and Increases Bone Density In Vivo

Table 1

Sequences of primers used in qPCR. OCN: Osteocalcin; BMP-2: Bone morphogenetic protein-2; ADPQ: Adiponectin, B2M: beta 2 microglobulin.

Gene	Primer	Sequence 5′-3′	Amplicon length (bp)	Accesion no.
OCN	F:	GGTGCAGACCTAGCAGACACCA	100	NM_001032298.2
	R:	CGCTGGGCTTGGCATCTGTAA
BMP-2	F:	CTACAGGGAGAACACCCGGA	280	NM_007553.3
	R:	GGGGAAGCAGCAACACTAGAA
ADPQ	F:	CCTGGAGAGAAGGGAGAGAAA	209	NM_009605.4
	R:	CGAATGGGTACATTGGGAAC
B2M	F:	CATACGCCTGCAGAGTTAAGCA	73	NM_009735.3
	R:	GATCACATGTCTCGATCCCAGTAG