Liver Cholesterol Overload Aggravates Obstructive Cholestasis by Inducing Oxidative Stress and Premature Death in Mice

Figure 1

A high cholesterol diet induces liver steatosis. (a) Macroscopic liver inspection of standard control Chow diet (Chow) and high cholesterol (HC) diet. (b) Free cholesterol determination by filipin. (c) Neutral lipid determined by Oil Red O staining (ORO) in Chow and HC liver sections. (d) Biochemistry determination in liver tissue of total cholesterol and triglycerides (TG) content. (e) Liver function tests: aspartate aminotransferase (AST) and alanine aminotransferase (ALT). Each column represents mean ± SEM of three independent experiments. Differences were considered significant at versus Chow. Images are representative of at least three independent experiments. Original magnification: 200x.

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