Liver Cholesterol Overload Aggravates Obstructive Cholestasis by Inducing Oxidative Stress and Premature Death in Mice

Figure 2

Cholesterol overload sensitizes to BDL-induced liver damage and aggravates obstructive jaundice. (a) Survival plot after BDL; at least nine animals were considered for the survival experiment. (b) Liver gross inspection and free cholesterol determination by filipin. Images are representative of at least three independent experiments. Original magnification for filliping staining: 100x. (c) Biochemical serum cholesterol and triglycerides (TG) determination. Liver function test: aspartate aminotransferase (AST); alanine aminotransferase (ALT); alkaline phosphatase (ALP); total bilirubin; direct bilirubin; serum total bile acids (TBA); and liver tissue TBA. Each column represents mean ± SEM of three independent experiments. Differences were considered significant at versus Sham Chow and versus Chow fed mice at the same time.

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