Liver Cholesterol Overload Aggravates Obstructive Cholestasis by Inducing Oxidative Stress and Premature Death in Mice

Figure 4

Cholesterol excess impairs cell proliferation. Tissue was obtained from Sham and three days after BDL animals, under (a-b) standard Chow control diet (Chow) or (c-d) high cholesterol (HC) diet, and Ki67 immunohistochemistry was assayed; arrows show positive cells (magnification at 100x). (e) Western blot and densitometric analysis of main cell cycle proteins. Actin was used as loading control; versus Sham Chow fed mice, versus Sham HC fed mice. Images are representative from at least four independent mice.

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