Functionalized Fullerene Increases NF-κB Activity and Blocks Genotoxic Effect of Oxidative Stress in Serum-Starving Human Embryo Lung Diploid Fibroblasts

Figure 5

F-828 shifts the levels of autophagy and mortality of HELFs. (a) (FCA): distribution of the cells treated with F-828 according to the FL1-BECLIN signal strength; dependence of the median values of FL1-BECLIN signals on the F-828 concentration. (b) (Fluorescence microscopy): changes in the levels of LC3-positive puncta in the HELFs cells treated with 0.5 µM of F-828. (c) (qRT-PCR): changes in the levels of mRNAs encoding BCL2A1, BCL2, BCL2L1, BIRC2, BIRC3, and BAX in HELFs. (d) (Fluorescence microscopy): morphological changes in the HELFs nuclei. Cells were stained with Hoechst 33342. Photo: HELFs in serum-free media (1 µM of F-828). Arrows point towards the cells, whose nuclei were entered by Hoechst 33342. The histogram presents the proportion of the cells with modified nuclei.

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