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Oxidative Medicine and Cellular Longevity
Volume 2017, Article ID 1289041, 11 pages
Research Article

The Role of Free Radicals in Hemolytic Toxicity Induced by Atmospheric-Pressure Plasma Jet

1Department of Electrical and Biological Physics, Kwangwoon University, Seoul 01897, Republic of Korea
2Department of Biomedical Engineering, College of Medicine, Kyung Hee University, Seoul 02447, Republic of Korea
3College of Veterinary Medicine, Chonbuk National University, 79 Gobong-Ro, Iksan, Jeonbuk 54596, Republic of Korea

Correspondence should be addressed to Eun Ha Choi; and Byoungchoo Park;

Received 20 January 2017; Revised 23 March 2017; Accepted 5 April 2017; Published 14 June 2017

Academic Editor: Tiziana Persichini

Copyright © 2017 Ku Youn Baik et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Atmospheric-pressure plasma (APP) has received attention due to its generation of various kinds of reactive oxygen/nitrogen species (ROS/RNS). The controllability, as well as the complexity, is one of the strong points of APP in various applications. For biological applications of this novel method, the cytotoxicity should be estimated at various levels. Herein, we suggest red blood cell (RBC) as a good cell model that is simpler than nucleated cells but much more complex than other lipid model systems. Air and N2 gases were compared to verify the main ROS/RNS in cytotoxicity, and microscopic and spectroscopic analyses were performed to estimate the damages induced on RBCs. The results shown here will provide basic information on APP-induced cytotoxicity at cellular and molecular levels.