Rat Liver Enzyme Release Depends on Blood Flow-Bearing Physical Forces Acting in Endothelium Glycocalyx rather than on Liver Damage
Table 2
Effect of flow rate and of glycocalyx modifiers in the content of NO and LP by-products (MDA) in perfusates from livers of sham-operated and PH animals.
Flow rate
mL⋅min−1⋅g−1 of liver
At 2
At 4
At 6
NO production (pmoles⋅min−1⋅g−1 of liver)
Sham + none
155 ± 19
235 ± 35
300 ± 51
Sham + hyaluronidase
132 ± 15
155 ± 22
160 ± 25
Sham + heparanase
162 ± 21
176 ± 35
142 ± 28
Sham + concanavalin A
125 ± 16
136 ± 23
137 ± 24
NO production (pmoles⋅min−1⋅g−1 of liver)
PH + none
257 ± 40
519 ± 73
353 ± 60
PH + hyaluronidase
151 ± 23
185 ± 26
183 ± 26
PH + heparanase
233 ± 42
389 ± 52
198 ± 39
PH + concanavalin A
215 ± 38
290 ± 53
207 ± 35
MDA production (nmoles⋅min−1⋅g−1 of liver)
Sham + none
14 ± 2
19 ± 3
32 ± 5
Sham + hyaluronidase
12 ± 2
14 ± 4
20 ± 4
Sham + heparanase
10 ± 2
15 ± 3
16 ± 4
Sham + concanavalin A
11 ± 2
13 ± 3
18 ± 3
MDA production (nmoles⋅min−1⋅g−1 of liver)
PH + none
23 ± 3
35 ± 4
65 ± 8
PH + hyaluronidase
16 ± 3
15 ± 4
40 ± 7
PH + heparanase
16 ± 3
22 ± 3
31 ± 5
PH + concanavalin A
19 ± 3
24 ± 4
45 ± 7
The results are the mean ± SE of five individual observations per experimental point for sham-operated control of partial hepatectomized (PH) rats, for nitric oxide (NO) and malondialdehyde (MDA) production. Statistical significance: against the control group, versus the PH group.
