Mitochondrial Respiration in Human Colorectal and Breast Cancer Clinical Material Is Regulated Differently

Table 1

Characterization of respiratory parameters of permeabilized tissue samples derived from patients with breast or colorectal cancer.

Parameters	HBC patients, [26]		HCC patients, [28]
	Tumor	Control	Tumor	Control
Vo	0.294 ± 0.024	0.004 ± 0.007	1.06 ± 0.14	0.82 ± 0.15
VADP	0.71 ± 0.06	0.055 ± 0.004	2.02 ± 0.21	1.39 ± 0.21
Vrot	0.34 ± 0.04	0.070 ± 0.015	0.91 ± 0.11	0.85 ± 0.14
VSucc	0.74 ± 0.10	0.076 ± 0.008	2.22 ± 0.26	1.33 ± 0.18
VANM	0.38 ± 0.04	0.071 ± 0.018	1.04 ± 0.09	0.69 ± 0.07
VCOX	2.36 ± 0.33	1.23 ± 0.18	6.59 ± 0.71	3.84 ± 0.58

Note: here, each data point is the mean ± SEM of respiratory values. Vo: basal respiration without ADP or ATP; V_ADP: ADP-stimulated respiration (final concentration 2 mM) in the presence of 5 mM glutamate and 2 mM malate (indicating the function of the respiratory chain complex I); Vrot: rates of respiration after addition of 50 μM rotenone (an inhibitor of complex I); V_Succ: ADP-stimulated respiration in the presence of rotenone and 10 mM succinate (to estimate the function of complex II); VANM: rates of respiration after addition 10 μM antimycin-A (an inhibitor of complex III); V_COX: rates of O₂ consumption in the presence of complex IV substrates (5 mM ascorbate jointly with 1 mM tetramethyl-p-phenylenediamine).