Impact of Atherosclerosis- and Diabetes-Related Dicarbonyls on Vascular Endothelial Permeability: A Comparative Assessment

Figure 6

MDA and MGO modify different proteins in EA.hy926 cells. Western blotting of total EA.hy926 lysates after treatment of the cells with MDA or MGO was performed using the moAb against MDA- and MGO-labelled proteins (see Materials and Methods for details). 1: the Coomassie R-250-stained gel resolving the total EA.hy926 cell lysate; 2 and 3: Western blot of the control (2) or MDA-treated cells (3) with 3G4 moAb, which recognizes the MDA-labelled proteins; 4 and 5: Western blot of the control (4) or MGO-treated cells (5) with 6D8 moAb, which recognizes MGO-labelled proteins. Position of the molecular weight markers (Precision Plus Protein Dual Color-prestained MW standards, Bio-Rad) is shown on the left. Protein bands in all lanes are aligned based on mobility of MW standards.