Research Article

The Stimulated Glycolytic Pathway Is Able to Maintain ATP Levels and Kinetic Patterns of Bovine Epididymal Sperm Subjected to Mitochondrial Uncoupling

Figure 1

Dose-response curve of FCCP concentrations (0.3, 1, 3, 10, 30, 60, and 100 μM) in sperm of bovine epididymal samples. Superscript numerals indicate the FCCP concentration used and their respective images. Mitochondrial depolarization of bovine spermatozoa at FCCP concentration of 0 μM (I), 10 μM (II), 30 μM (III), and 60 μM (IV). Arrows indicate mitochondria labeled with the TMRE fluorescent probe at different percentages of mitochondrial depolarization. 400x magnification.