Apple Flavonoids Suppress Carcinogen-Induced DNA Damage in Normal Human Bronchial Epithelial Cells

Figure 3

(a) BEAS-2B cells were exposed to either carcinogens alone or in combination with pretreatment of AF4 followed by immunofluorescence staining with γ-H2AX antibody and were captured by epifluorescence microscopy at 100x magnification. Nuclei were stained as blue and γ-H2AX foci (S 139) appeared as red. The image shown represents cells from three independent experiments. (b) Quantification of focus/nucleus ratio was calculated for each sample from at least 50 cells. indicated statistical difference at .

(a)

(b)