HO-1 Is Essential for Tetrahydroxystilbene Glucoside Mediated Mitochondrial Biogenesis and Anti-Inflammation Process in LPS-Treated RAW264.7 Macrophages
TSG reduced RAW264.7 cells activation and proinflammatory cytokines release induced by LPS. RAW264.7 cells were exposed to various concentrations of TSG (0–480 μM) for 6 hours. (a) Cell viability was analyzed by WST assay. (b) Annexin V-PI method was used to detect the cell death rate. (c-d) RAW264.7 cells were exposed to 1 μg/mL LPS for 12 hours with or without TSG (120 μM) pretreatment for 6 hours. Then, the morphological change was captured under a microscope. Bar = 50 μm (×30). The percentage of activated cells in total cell population was calculated. (e-f) In the presence and absence of TSG (0, 30, 60, and 120 μM) or Dex (100 μM) for 6 hours, RAW264.7 macrophages were stimulated with 1 μg/mL LPS for 12 hours. The concentration of TNF- and IL-6 production in the culture medium were assessed by specific ELISA kits. All data were expressed as the means ± SEM of three independent experiments. and versus control; # versus LPS treatment group.