HO-1 Is Essential for Tetrahydroxystilbene Glucoside Mediated Mitochondrial Biogenesis and Anti-Inflammation Process in LPS-Treated RAW264.7 Macrophages
TSG stimulated HO-1 gene and protein expression via an NRF2 dependent pathway. (a) After RAW264.7 cells were stimulated with TSG (120 μM) for 6 hour, the relative mRNA levels of HO-1, CAT, SOD1, SOD2, NQO-1, and GPX-1 were evaluated by Real-Time PCR. (b) RAW264.7 cells were treated with various concentrations (0, 30, 60, and 120 μM) of TSG for indicated times (0, 1, 2, 4, and 6 h), and then HO-1 protein level was detected by western blot analysis. (c) RAW264.7 cells were exposed to TSG (120 μM) for 6 hours in the presence and absence of Brusatol (50 μM). Western blot analysis assessed NRF2 and HO-1 protein expression in cell lysates. (d) The relative HO-1 protein level was analyzed by densitometry. Data were expressed as the mean ± SEM of triplicate independent experiments (). versus control group; # versus TSG treatment group.