HO-1 Is Essential for Tetrahydroxystilbene Glucoside Mediated Mitochondrial Biogenesis and Anti-Inflammation Process in LPS-Treated RAW264.7 Macrophages

Figure 5

The mitochondrial biogenesis induced by TSG was regulated by activation of HO-1. RAW264.7 cells were treated with TSG (120 μM) for 6 hours in the presence and absence of ZnPP (20 μM). (a) After treatment, mRNA expressions of PGC-1, NRF1, and TFAM were detected by Real-Time PCR. (b) Western blot analysis was used to assess the expression of mitochondrial complex IV in whole cell lysates. (c) The relative COX-IV protein level was analyzed by densitometry. (d) Quantitative PCR was performed to evaluate the mtDNA content with beta2-microglobulin and cytochrome b served as probes specific for nuclear DNA and mtDNA. Relative ratios of mtDNA and nDNA contents were analyzed. All measurements were expressed as the mean ± SEM of triplicate independent experiments (). and versus control group; ^# versus TSG treatment group.

(a)

(b)

(c)

(d)