YiQiFuMai Powder Injection Protects against Ischemic Stroke via Inhibiting Neuronal Apoptosis and PKCδ/Drp1-Mediated Excessive Mitochondrial Fission

Figure 5

YQFM attenuates H₂O₂-induced excessive mitochondrial fission in neurons. Neurons were pretreated with YQFM (400 μg/ml), mdivi-1 (25 μM), or NAC (500 μM) for 6 h before exposure of H₂O₂ (100 μM) for 6 h. (a) Mitochondrial morphology was viewed by MitoTracker Deep Red FM using confocal microscopy. Bar = 5 μm. (b) Quantitative analysis of percentage of fragmented mitochondria. (c) Mitochondrial localization of Drp1 (green) and mitochondrial morphology (red) were imaged using confocal microscopy. Bar = 5 μm. (d) Colocalization of Drp1 and MitoTracker was quantified on the basis of Manders’ overlap coefficients. (e) Expression of Drp1 and p-Drp1 (Ser616) were detected by Western blot. Data were presented as mean ± SD from three independent experiments. versus control, versus H₂O₂, versus H₂O₂.

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