Increased Circulating Levels of Interleukin-6 Induce Perturbation in Redox-Regulated Signaling Cascades in Muscle of Dystrophic Mice

Figure 2

Nrf2 antioxidant genes are differently regulated in the prenecrotic dystrophic muscle. (a) Real-time PCR analysis of Nrf2-dependent genes (SOD1, SOD2, CAT-1, Gpx1, GCL, and NQO1) performed on the diaphragm muscle of 2-week-old WT, mdx, and mdx/IL6 mice. Values are reported as fold change in expression relative to the calibrator (WT, horizontal dot line) and represent mean ± SEM; to 5 per group. value by unpaired statistical tests. ^{^}, ^{^^}. Analysis of HO-1 mRNA (b), IL-6 mRNA (c), TNFα mRNA (d), IL-1β mRNA (e), IL-10 mRNA (f), and IL6rα mRNA (g) expressions in the diaphragm of 2-week-old WT, mdx, and mdx/IL6 mice. Data are presented as the mean ± SEM; to 6 mice per group. , , and compared to WT mice; ^#, ^## between mdx and mdx/IL6 littermates (by ANOVA). (h) Densitometric analysis (left panel) and representative western blot (right panel) for NFκB active (NFκB p65) and total (NFκB TOT) protein expression in the diaphragm muscle of indicated genotypes. Values are reported as mean ± SEM; to 7 per group. p using Student’s two-tailed t-test. In (h), gels were simultaneously run under same experimental conditions.

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