Increased Circulating Levels of Interleukin-6 Induce Perturbation in Redox-Regulated Signaling Cascades in Muscle of Dystrophic Mice

Figure 3

X-ROS signaling is altered in the prenecrotic dystrophic diaphragm muscle and increased during the progression of pathology. Densitometric analysis (upper panels) and representative images (bottom panels) of western blot analysis for the expression of α-tubulin (a), β-tubulin (b), and Glu-tubulin (c) proteins in the diaphragm muscle of 2-week-old WT, mdx, and mdx/IL6 mice. Values represent mean ± SEM; to 7 mice per group. value by ANOVA. X-ROS signaling components were analysed by western blot (left panels show representative images) at later stages of pathology in 4-week-old (d) and 24-week-old (e) diaphragm muscle from WT, mdx, and mdx/IL6 mice. Values are reported as protein content relative to age-matched WT (horizontal dot line) and represent mean ± SEM; to 7 mice per group. ^{^}, ^{^^}, and ^{^^^} compared to WT mice; , with respect to mdx littermates (by ANOVA). Temporal progression of gp91phox (f), α-tubulin (g), β-tubulin (h), and Glu-tubulin (i) proteins between 4 weeks (4 w) and 24 weeks (24 w) of age in the diaphragm muscles of indicated genotypes. Graphs show an increase of the expression levels of tubulin subunits and of gp91phox protein during the progression of pathology. Values represent mean ± SEM. , , and by ANOVA.

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