Increased Circulating Levels of Interleukin-6 Induce Perturbation in Redox-Regulated Signaling Cascades in Muscle of Dystrophic Mice

Figure 4

Time course analysis of Nrf2-dependent antioxidant response in dystrophic mice. Real-time PCR analysis performed on diaphragm muscles from wild-type (WT), mdx, and mdx/IL6 mice at 4 (a) and 24 (c) weeks of age for the expression of Nrf2-dependent genes involved in the antioxidant response. Values are reported as fold change in the expression relative to the calibrator (WT, horizontal dot line) and represent mean ± SEM; to 6 mice per group. ^{^}, ^{^^} compared to WT mice; , with respect to mdx littermates (by ANOVA). Nrf2 protein expression was evaluated by western blot analysis (right panels show representative images) in 4-week-old (b) and 24-week-old (d) diaphragms of indicated genotypes. Densitometric analyses (left panels) are expressed as values relative to age-matched mdx and represent mean ± SEM; to 5 mice per group. value using Student’s two-tailed t-test.

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