The Transcription Factor Bach1 Suppresses the Developmental Angiogenesis of Zebrafish

Figure 2

Bach1 overexpression induces vascular defects in zebrafish embryos. (a) Zebrafish vasculature was evaluated via fluorescent images (C-D) or confocal images (E and F) in normal 32-hpf Tg(fli1:EGFP) zebrafish embryos (A, C and E) and in embryos that had been injected with 200 pg of Bach1 mRNA (B, D and F) at the single-cell stage. Bach1 mRNA injections disrupted formation of the intersegmental vessels (ISV) (arrow) and the dorsal longitudinal anastomotic vessels (DLAV) (asterisks). (A–D) Scale bar: 100 μm. (E and F) Scale bar: 50 μm. (b) Bach1 mRNA levels were evaluated in normal and Bach1-mRNA–injected embryos via quantitative real-time RT-PCR (; versus Control). (c) Embryos with defects in ISV and DLAV were counted and expressed as a percentage of the total number of embryos in each experimental group (; versus Control).

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