The Transcription Factor Bach1 Suppresses the Developmental Angiogenesis of Zebrafish

Figure 3

Bach1 suppresses endogenous Wnt/β-catenin signaling and exogenous Wnt8a stimulated VEGF and IL-8 gene expression in zebrafish. (a) Representative fluorescent images of 36-hpf Tg(TOP:GFP) transgenic zebrafish embryos that had been injected with or without Bach1 mRNA. (b) The fluorescence intensity of GFP was quantified and expressed as the as the ratio of measurements Bach1 mRNA and Control group (; versus Control). (c) IL-8, VEGF₁₆₅, and Bach1 mRNA levels were evaluated in 32-hpf Tg(hsp:wnt8a) embryos following Bach1 mRNA injections at the one cell stage and Wnt pathway activation (heat-shock) at 24 hpf (; , versus Control; versus Wnt8a).

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