The Transcription Factor Bach1 Suppresses the Developmental Angiogenesis of Zebrafish

Figure 6

Exogenous administration of VEGF or IL-8 partially rescued Bach1-driven antiangiogenic response in HUVECs. (a) HUVECs were transfected with AdGFP or AdBach1 for 24 hours and then incubated with or without VEGF (50 ng/mL) or IL-8 (50 ng/mL) for 24 hours. Endothelial tube formation assay in Matrigel was performed in presence of VEGF or IL-8. Tube length was quantified and expressed as the fold change relative to AdGFP (; , ). Scale bar: 500 μm. (b) Bach1 and β-actin protein levels were evaluated via Western blot.

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