Activation of Endocannabinoid Receptor 2 as a Mechanism of Propofol Pretreatment-Induced Cardioprotection against Ischemia-Reperfusion Injury in Rats

Figure 2

Effects of propofol conditioning on cardiomyocyte endocannabinoid release. (a) Cell culture media AEA concentrations among groups. (b) Cell culture media 2-AG concentrations among groups. In the cardiomyocyte hypoxia/reoxygenation (H/R) injury model, propofol conditioning was achieved by continuous incubation at a concentration of 50 μM from 1 hour prior to hypoxia until the end of hypoxia. Cell culture media were collected at 10 minutes after the beginning of propofol conditioning and at 0, 1, 2, and 4 hours after hypoxia. Endocannabinoids including AEA and 2-AG were detected by LC/LC-MS. The results showed that propofol conditioning and I/R enhanced cardiac endocannabinoid release in vivo. per group for each time point. : ; : versus control. #: ; ##: versus propofol. ‡: ; ‡‡: versus baseline. &: ; &&: versus 10 mins after the beginning of propofol exposure. §: ; §§: versus the end of hypoxia. ; ¶¶: versus 1 hr after hypoxia. ; ££: versus 2 hr after hypoxia. H/R, hypoxia/reoxygenation.

(a) Cell culture media AEA

(b) Cell culture media 2-AG