Resveratrol Modulation of Protein Expression in parkin-Mutant Human Skin Fibroblasts: A Proteomic Approach

Figure 4

Western blot analysis of CTR and P1 fibroblasts treated with resveratrol. CTR and P1 cells were treated with resveratrol at the concentration of 25 μM for 24 h. Proteins were separated by SDS-PAGE and probed with the following antibodies: ezrin (1 : 2000, Santa Cruz, sc-20773), alpha B crystallin (1 : 1000, Proteintech, 15808-1-AP), peroredoxin-1 (1 : 2000, Santa Cruz, sc-7381), peroredoxin-2 (1 : 2000, Santa Cruz, sc-33572), peroredoxin-6 (1 : 2000, Santa Cruz, sc-393024), and heat-shock protein (HSP70) (1 : 2000, Sigma, H51747). α-Tubulin was used as loading control (1 : 4000, Santa Cruz, sc-23948). This image is representative of three independent experiments.

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