Resveratrol Modulation of Protein Expression in parkin-Mutant Human Skin Fibroblasts: A Proteomic Approach

Figure 5

Effect of resveratrol treatment on GSH and GSSG content in patient and control cells. Primary fibroblasts from patient (P1) and control fibroblasts (CTR) were grown as specified in Materials and Methods. As indicated, cells were incubated with 25 μM resveratrol for 24 hours (Res). GSH and GSSG content were determined in total cellular lysate. (a) The histogram represents the mean values of GSH basal level ± SEM of different experiments (). (b, c) Effect of Res treatment on GSH content in CTR and P1 cells. The values are means ± SEM of different experiments (). (d) The histogram represents the mean values of normalized GSSG/GSH ratio ± SEM of different experiments (). (e, f) Effect of Res treatment on GSSG/GSH ratio in CTR and P1 cells. The values are means ± SEM of different experiments (). value was determined by Student’s t-test, , .

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