The Preconditioning of Berberine Suppresses Hydrogen Peroxide-Induced Premature Senescence via Regulation of Sirtuin 1

Figure 1

Antisenescence effect of BBR on H₂O₂-induced premature senescence in human diploid fibroblasts by β-galactosidase staining. 2BS cells were pretreated with different concentrations of BBR for 12 hr before exposure to 200 μmol/L H₂O₂. For senescence assay, cells were cultured for four days with new fresh DMEM medium. On the fifth day, the blue precipitate can be seen by X-gal dye in senescent cells as the Materials and Methods mentioned. (a), (b), and (c) Pretreatment with 6, 12, and 20 μmol/L BBR, respectively. (d) H₂O₂ treatment alone. (e) Untreated control. (f) The positive rates of SA-β-gal were calculated by counting the blue-dyed cells with a total of 200 cells at each visual field. , . The results are representative of three separate experiments.

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