The Preconditioning of Berberine Suppresses Hydrogen Peroxide-Induced Premature Senescence via Regulation of Sirtuin 1

Figure 2

Cell cycle arrest and protein expression of H₂O₂-induced premature senescence in human diploid fibroblasts. 2BS cells were exposed to a sublethal concentration of 200 μmol/L H₂O₂, or pretreated with 12 μmol/L BBR for 12 hr before exposure. (a), (b) The influence of BBR in H₂O₂-induced cell cycle distribution. (c) The changes of SIRT1 expression and phosphorylation of Chk2 in H₂O₂-exposed human diploid fibroblasts. (d) Relative expression levels of Sirt1 and phospho-Chk2 in H₂O₂-exposed human diploid fibroblasts by gray analysis. , . The results are representative of three separate experiments.

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