The Preconditioning of Berberine Suppresses Hydrogen Peroxide-Induced Premature Senescence via Regulation of Sirtuin 1

Figure 3

Protective effect of BBR on H₂O₂-induced mitochondrial membrane potential (Δψm) loss in human diploid fibroblasts. 2BS cells were pretreated with or without 12 μmol/L BBR for 12 hr before a 2 hr exposure to 200 μmol/L H₂O₂ and then were stained with Rhodamin123. (a) The diagram from flow cytometry test. M1 represents the percentage of Δψm loss. (b) Histogram of the mean fluorescence intensity of M1. , . The results are representative of three separate experiments.

(a)

(b)