The Preconditioning of Berberine Suppresses Hydrogen Peroxide-Induced Premature Senescence via Regulation of Sirtuin 1

Figure 5

Protective effect of BBR on H₂O₂-induced reduction of SIRT1 expression and augment of phosphorylation of Chk2 in human diploid fibroblasts. 2BS cells were pretreated with 12 μmol/L BBR for 12 hr before a 2 hr or 4 hr exposure to 200 μmol/L H₂O₂. (a) Sirt1 and phospho-Chk2 was detected by western blotting. (b) Relative expression levels of Sirt1 and phospho-Chk2 in cells with different treatment by gray analysis. , . The results are representative of three separate experiments.

(a)

(b)