2-Deoxy-D-glucose Restore Glucocorticoid Sensitivity in Acute Lymphoblastic Leukemia via Modification of N-Linked Glycosylation in an Oxygen Tension-Independent Manner
Glucose consumption in a panel of ch-ALL cell lines. The absolute glucose levels were measured in the cell media after incubation with MP, 2-DG, or combinatorial treatment. Kinetic measurements of glucose uptake were performed; only data from 96 h are shown. (a) SUP-B15, (b) SD1, and (c) REH cell lines were treated with increasing concentration of MP (left graph) and 2-DG (right graph). RPMI and IMDM controls represent the amount of glucose present in the culture medium incubated for 96 h in the absence of treatment. The effects of combined drug treatments on glucose levels are shown in (d), (e), and (f) for SUP-B15, SD1, and REH, respectively. The conversion of glucose to glucose-6-phosphoglucoronate (G6P) and NADH was evaluated. The concentration of NADH was measured. Graphs represent the SD of the mean of at least two independent experiments (). using two-way analysis of variance (ANOVA) using Bonferroni’s multiple comparison test.
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