2-Deoxy-D-glucose Restore Glucocorticoid Sensitivity in Acute Lymphoblastic Leukemia via Modification of N-Linked Glycosylation in an Oxygen Tension-Independent Manner

Figure 5

Gene expression analysis. REH transfection with siRNA was performed using nucleofection and gene expression was assessed. (a) Expression of HKII after silencing was measured in REH cells line at the mRNA level (24 h) and (b) at the protein level (48 h). The effects of (c) 2-DG or (d) MP on cell viability were measured in the presence or absence of HKII expression. The fold change was calculated via normalization to the scrambled control. Error bars represent the SD of the mean of at least three independent experiments ().

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