2-Deoxy-D-glucose Restore Glucocorticoid Sensitivity in Acute Lymphoblastic Leukemia via Modification of N-Linked Glycosylation in an Oxygen Tension-Independent Manner
Metabolic profiles upon treatment with 2-DG and MP in ch-ALL. (a) Reversal effects of 2-DG on cell viability by exogenous mannose (2.5 mM). (b) Principal component analysis (PCA) score plot of SUP-B15 (●), SD1 (▲), and REH (■). Representative data are shown; the size of each condition is proportional to the treatment exposure. (c) The hierarchical clustering heat map shows consistent metabolic changes of ch-ALL cell lines upon 48 h of combinatorial treatment. (d) Enrichment in metabolites involves in the synthesis of the UDP-N-acetyl-glucosamine pathway in a matrix of 60 columns for 4 treatments (ctrl, 2-DG, MP, and 2-DG+MP) and 5 time points (0, 6, 12, 18, 24, and 48 h). Error bars represent the SD of the mean of at least three independent experiments (). , , , two-way analysis of variance (ANOVA) using Bonferroni’s multiple comparison test.
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