2-Deoxy-D-glucose Restore Glucocorticoid Sensitivity in Acute Lymphoblastic Leukemia via Modification of N-Linked Glycosylation in an Oxygen Tension-Independent Manner

Figure 7

UPR induction by 2-DG in sensitive and resistant ch-ALL cell lines. (a) GRP78/Bip and (b) CHOP mRNA expression was assessed by real-time RT-PCR (SYBR-green) in leukemia cells treated with 2-DG (dark grey bar), MP (light grey bar), 2-DG+MP (grey striped bar), or 2-DG+Mann (white striped bar). Error bars represent the SD of the mean of at least three independent experiments (). β-Actin was used as a control to normalize the samples. (ns), using two-way analysis of variance (ANOVA) with Bonferroni’s multiple comparison test.

(a)

(b)