An Extract from the Plant Deschampsia antarctica Protects Fibroblasts from Senescence Induced by Hydrogen Peroxide

Figure 2

Effect of EDA in SA-β-Gal levels and protein contents of Sirt1 and Lmn A/C of HFF cells. (a) SA-β-Gal levels were measured as nmol of fluorescein by number of cells in control (C, untreated EDA cells) and EDA-treated cells with 0.3, 0.5, and 1 mg/mL. (b) Densitogram of Sirt1 in control and EDA-treated cells. (c) Western blot of Sirt1 in control and EDA-treated cells. (d) Densitogram (upper panel) and Western blot for LmnA/C (lower panel) in control and EDA-treated cells. Capital letters indicate statistical comparative analysis among LmnA data and lowercase letters among LmnC. Act-C is used as loading control. The time of EDA treatment was 24 h after seeding during 24 h (Cond 1), 48 h after seeding during 24 h (Cond 2), and 24 h after seeding during 48 h (Cond 3). See more details in Supplemental Figure S1. Each bar represents the mean ± S.E. of three replicates from three independent experiments, and samples that do not have a common letter are significantly different in each condition by Duncan’s test at .

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