An Extract from the Plant Deschampsia antarctica Protects Fibroblasts from Senescence Induced by Hydrogen Peroxide

Figure 3

Effect of H₂O₂ in cell proliferation and survival rate of HFF cells. Cell proliferation and survival rate of control (H₂O₂-unexposed cells) and cells exposed to 100 and 200 μM H₂O₂ during 30 min, 1 h, and 2 h. Measures were done at 24 h (a), 48 h (b), and 72 h (c) after H₂O₂ recovery. (d) Densitogram (left panel) and Western blot (right panel) of PCNA in controls (C13, cells of 13 PD (HFF) and C45, cells of 45 PD (SHFF)), and cells exposed to 100, 150, and 200 μM H₂O₂ during 2 h and after 24 h of recovery. Capital letters indicate statistical comparative analysis among data from 100 μM H₂O₂ treatment and lowercase letters among the 200 μM H₂O₂ treatment. Each bar represents the mean ± S.E. of three replicates from three independent experiments, and samples that do not have a common letter are significantly different by Duncan’s test at .

(a) 24 h

(b) 48 h

(c) 72 h

(d) 24 h