An Extract from the Plant Deschampsia antarctica Protects Fibroblasts from Senescence Induced by Hydrogen Peroxide

Figure 6

Effect of EDA in cell proliferation and viability of SIPSF cells. (a) Cell proliferation and (b) survival rate of control (C, unexposed HFF cells, neither EDA nor H₂O₂), control 200 μM H₂O₂-exposed cells (C + H₂O₂), and H₂O₂-exposed cells treated with 0.3, 0.5, and 1 mg/mL EDA. (c) Densitogram (upper panel) and Western blot (lower panel) of PCNA in control (C, unexposed HFF cells, neither EDA nor H₂O₂), control 200 μM H₂O₂-exposed cells (C + H₂O₂), and H₂O₂-exposed cells treated with 0.3, 0.5, and 1 mg/mL EDA. The time of EDA treatment was 24 h before H₂O₂ exposition (PRE), just after H₂O₂ exposition (POST), and after and before H₂O₂ exposition (PRE-POST). Each bar represents the mean ± S.E. of three replicates from three independent experiments, and samples that do not have a common letter are significantly different in each condition (PRE, POST, and PRE-POST) by Duncan’s test at .

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