An Extract from the Plant Deschampsia antarctica Protects Fibroblasts from Senescence Induced by Hydrogen Peroxide

Figure 8

Effect of EDA in protein levels of Sirt1 and LmnA/C of SIPSF cells. (a) Densitogram (upper panel) and Western blot (lower panel) of Sirt1 and (b) LmnA/C in control (C, unexposed HFF cells), control 200 μM H₂O₂-exposed cells (C + H₂O₂), and H₂O₂-exposed cells treated with 0.3, 0.5, and 1 mg/mL EDA. The time of EDA treatment was 24 h before H₂O₂ exposition (PRE), just after H₂O₂ exposition (POST), and after and before H₂O₂ exposition (PRE-POST). Capital letters indicate statistical comparative analysis among LmnA data and lowercase letters among LmnC. Each bar represents the mean ± S.E. of three replicates from three independent experiments, and samples that do not have a common letter are significantly different in each condition (PRE, POST, and PRE-POST) by Duncan’s test at .

(a)

(b)