An Extract from the Plant Deschampsia antarctica Protects Fibroblasts from Senescence Induced by Hydrogen Peroxide

Figure 9

Effect of EDA over contents of Trx2 of HFF and SIPSF cells. (a) Densitogram (upper panel) and Western blot (lower panel) of Trx2 in HFF cells treated with different EDA doses. The time of EDA treatment was 24 h after seeding during 24 h (Cond1), 48 h after seeding during 24 h (Cond2), and 24 h after seeding during 48 h (Cond3). (b) Densitogram (upper panel) and Western blot (lower panel) of Trx2 in control (C, unexposed HFF cells), control 200 μM H₂O₂-exposed cells (C + H₂O₂), and H₂O₂-exposed cells treated with 0.3, 0.5, and 1 mg/mL EDA. The time of EDA treatment was 24 h before H₂O₂ exposition (PRE), just after H₂O₂ exposition (POST), and after and before H₂O₂ exposition (PRE-POST). Each bar represents the mean ± S.E. of three replicates from three independent experiments, and samples that do not have a common letter are significantly different in each condition (Cond (a) or PRE, POST, and PRE-POST (b)) by Duncan’s test at .

(a)

(b)