VLDL Induced Modulation of Nitric Oxide Signalling and Cell Redox Homeostasis in HUVEC

Figure 2

eNOS protein expression and evaluation of uncoupling in response to VLDL by Western blot. HUVEC were incubated 24 h with (75 and 140 μg/mL) n-VLDL or ox-VLDL and then assayed by Western blot with anti-eNOS antibodies (see Materials and Methods). (a) Levels of eNOS protein after n-VLDL or ox-VLDL treatment. Densitometric values are shown as fold change versus the eNOS protein expressed by control cells. Data ± SD, n. of biological experiments = 8. values were considered statistically significant by ANOVA. Inset: Western blot pattern of eNOS after 24 h incubation with n-VLDL or ox-VLDL; α-tubulin as reference (α-tub). (b) Phosphorylation of eNOS at Ser-1177 and Thr-495 in response to n-VLDL or ox-VLDL (see Materials and Methods). Data ± SD, n. of biological experiments = 4. values were considered statistically significant by ANOVA. Inset: Western blot pattern of eNOS (P-S1177) and eNOS (P-T495) after 24 h incubation with n-VLDL or ox-VLDL.

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