Protective Effect of Icariin on the Development of Preimplantation Mouse Embryos against Hydrogen Peroxide-Induced Oxidative Injury

Table 2

The effects of different concentrations of ICA after transient treatment of 60 μM H₂O₂ on the development of KM mouse 1-cell embryos in vitro.

Group	1-cell embryos	2-cell embryos (%)	4-cell embryos (%)	Blastocysts (%)
KSOM	119	115 (96.64)	109 (91.60)	103 (86.55)
60 μM H2O2	108	55 (50.93)	41 (37.96)	31 (28.70)
60 μM H2O2+ 10 μM ICA	110	57 (51.82)	46 (41.82)	36 (32.73)
60 μM H2O2+ 20 μM ICA	120	70 (58.33)	59 (49.17)	50 (41.67)
60 μM H2O2+ 40 μM ICA	118	94 (79.66)	79 (66.95)	74 (62.71)
60 μM H2O2+ 80 μM ICA	109	64 (58.72)	51 (46.79)	47 (43.12)

Note: The percentage is based on the numbers of 1-cell embryos. Differences between the groups were calculated using the χ²-test. , versus the 60 μM H₂O₂ group.